Figure 6.

eGFP⁺ neurons in the spinal cord of UCHL1-eGFP mouse display electrophysiological characteristics of SMN. A, Lumbar spinal cord showing the location of eGFP⁺ neurons that are patch-clamped (top panel), targeted for recording (second panel) and filled with Texas Red dextran (third and bottom panel). B, eGFP⁺ neurons of UCHL1-eGFP mice (blue), eGFP⁺ neurons in ventral horn in the HB9-eGFP mice (gray), and WT motor neurons identified based on location and size (red) are used for recording. C, Representative traces showing subthreshold properties. D, Sag, current, RMP, rebound current (as percentage of steady state), and whole-cell input resistance. E, Representative traces showing suprathreshold firing properties. F, Voltage threshold, FI slope, AP height, and width show comparable properties between Hb9-eGFP and UCHL1-eGFP neurons, whereas a small difference is observed with WT neurons in FI slope measurements. One-way ANOVA followed Student's t test: *p < 0.001. Error bars indicate SEM. Scale bars (from top to bottom): A, 250 μm, 100 μm.