Fig. 6.
Profiles of the OTP obtained with n-SASLs at 37°C for (A) cortical and (B) nuclear intact membranes. Profiles are reported for bulk plus boundary lipids and for trapped lipids. OTP (mean values and standard deviations) obtained with 12-SASL from three different preparations of membranes from eyes of three different donors are 1.84 ±0.65 µs−1 for bulk plus boundary lipids and 0.31 ±0.12 µs−1 for trapped lipids in cortex and are 1.61 ±0.60 µs−1 for bulk plus boundary lipids and 0.22 ±0.12 µs−1 for trapped lipids in nucleus. Values obtained with ASL in domains of cortical and nuclear membranes are also included. Superimposed are profiles for cortical (A) and nuclear (B) lens lipid membranes (broken (bulk domain) and dotted (CBD) lines drawn based on data presented in (Mainali et al., 2013b)). Approximate localizations of the nitroxide moieties of spin labels are indicated by arrows.