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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: J Mol Biol. 2013 Dec 29;426(6):1308–1321. doi: 10.1016/j.jmb.2013.12.023

Figure 4. NA co-immunoprecipitates with tetherin and the maturation state of the bound tetherin correlates with NA budding competence.

Figure 4

293T (panels A and B) or Cos-1 cells (panel C), were transfected with DNA encoding NA alone or co-transfected with plasmids encoding NA and tetherin, as indicated. Approximately 48–60 hours post-transfection, the cells were harvested, lysates prepared, and NA proteins immunoprecipitated. Panel A, NA and/or tetherin in immunoprecipitates was detected by western blotting with either anti-NA or anti-HA-tag (tetherin) antibodies. Panel B, Equivalent amounts of immunoprecipitated sample was either left untreated (NAT) or treated with either PNGase or EndoH prior to western blot analysis.