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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: J Mol Biol. 2013 Dec 29;426(6):1308–1321. doi: 10.1016/j.jmb.2013.12.023

Figure 5. Tetherin is induced upon WT influenza virus infection.

Figure 5

Panel A, A549 cells were mock-infected or infected with the indicated viruses at an MOI of 0.05. 24 hours post-infection, cells were harvested and tetherin mRNA levels were quantified by real-time PCR. Panel B, A549 cells were infected as in A, but were lysed directly in 2× SDS sample buffer and analyzed by western blot and non-reducing SDS-PAGE for the indicated proteins. The image shows a single western blot from which superfluous intervening lanes have been cropped.