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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: J Mol Biol. 2013 Dec 29;426(6):1308–1321. doi: 10.1016/j.jmb.2013.12.023

Figure 8. Late events in the influenza life cycle are specifically targeted by tetherin.

Figure 8

WT and stable, tetherin shRNA-expressing, NCI-H196 cells were infected at an MOI of 1.0 and virus present in supernatants was quantified by plaque assay (panel A) or purified over a sucrose cushion for western blot analysis (panel B). As a loading control for tissue culture supernatants, equal amounts of WT Adenovirus (dl309) were added to each sample prior to centrifugation. Whole cell extracts were also prepared and analyzed. Anti-PDI antibody was used as a loading control for whole cell extracts (WCEs).