Figure 4.

Meiotic Irregularities in the Syn × Nat F2 Plants.

(A) to (C) FISH observations of meiotic spreads of developing microspores from F2 individuals. From left to right: Overlay, 4′,6-diamidino-2-phenylindole staining in blue, At-cen labeled FISH in red, Aa-cen labeled FISH in green.

(A) Anaphase I, example of a regular meiosis.

(B) Anaphase I, one potential laggard from an arenosa chromosome is observed.

(C) Anaphase I, an arenosa chromosomal bridge is observed.

(D) Correlation between departure from expected chromosome number and the percentage of viable pollen in the F2 population. The regression P value and R² value are indicated.