Figure 7.

JAZ4 and IAA29 Competitively Interact with WRKY57.

(A) The Domain II of IAA29 is necessary for interaction with WRKY57. Left: Diagram of full-length and truncated IAA29 constructs with specific deletions. Right: Interactions were indicated by the ability of yeast cells to grow on synthetic dropout medium lacking Leu, Trp, His, and Ade. The empty pGADT7 prey vector was used as a negative control.

(B) The ZIM domain of JAZ4 is required for interaction with WRKY57. Left: Diagram of full-length and truncated JAZ4 constructs with specific deletions. Right: Interactions were indicated by the ability of yeast cells to grow on synthetic dropout medium lacking Leu, Trp, His, and Ade. The empty pGADT7 prey vector was used as a negative control.

(C) The zinc-finger domain of WRKY57 is necessary for interaction with JAZs and IAA29. Left: Diagram of full-length and truncated WRKY57 constructs with specific deletions. Right: Interactions were indicated by the ability of yeast cells to grow on synthetic dropout medium lacking Leu, Trp, His, and Ade. The empty pGBKT7 prey vector was used as a negative control. In (A) to (C), AD and BD refer to the pGADT7 and pGBKT7 vector, respectively.

(D) and (E) Competition between JAZ4 and IAA29 for interaction with WRKY57. DAPI, 4′,6-diamidino-2-phenylindole.