Protein Blotting Analysis of WRKY57 Protein Levels.
Four-week-old ProWRKY57:Myc-WRKY57 transgenic plants were used for protein gel blotting analysis upon treatment with water (mock) (A), IAA
(B), MeJA
(C), or MeJA+MG132 (D) in the indicated time periods. The immunoblot was detected with β-tubulin antibody as a protein-loading control.