Figure 5.

ChIP Confirms DEX-Dependent Association of KAN-GR with the Promoters of KANT Genes.

ChIP was performed on 9-d-old transgenic Arabidopsis seedlings using antibodies specific for GR. Immunoprecipitated genomic DNA from mock (M) and DEX (D) treated KAN-GR and wild-type control seedlings (Supplemental Figure 4) was amplified with primers specific for the indicated promoters. Fold enrichment was calculated by normalizing PCR product intensities to a negative control, the RIBOSOMAL PROTEIN L4D (RPL4D) coding region, followed by calculating the ratio DEX IP/input to mock IP/input. The mean of at least two independent IP experiments (Supplemental Table 2) with technical replicates is reported as fold enrichment. Schematics of the gene promoters are shown with the positions and orientations of KBX sites indicated by < or > and the amplified region represented by a gray bar.