Mutations in EIN3 and EIL1 Block the Exaggerated Hook Curvature of myc2 and myc2 myc3 myc4.
(A) The hook phenotypes of 4-d-old etiolated Arabidopsis
Col-0 (WT), myc2-2 (myc2), jin1-2 myc3 myc4 (myc2/3/4), myc2-2 ein3 eil1 (myc2 ein3 eil1), jin1-2 myc3 myc4 ein3 eil1 (myc2/3/4 ein3 eil1), and ein3 eil1 grown in the dark on MS medium supplied without (Mock) or with 5 μM MeJA (JA), 10 μM ACC, or 10 μM ACC plus 5 μM MeJA (ACC+JA).
(B) Real-time PCR analysis for HLS1 in the indicated 4-d-old etiolated seedlings. Actin8 was used as the internal control. Data are means (±sd) of three biological replicates. Lowercase letters indicate significant differences by one-way ANOVA analysis with SAS software (P < 0.05).
(C) Real-time PCR analysis for HLS1 in the indicated 4-d-old etiolated seedlings treated with mock, 100 μM MeJA (JA), 100 μM ACC, or 100 μM ACC plus 100 μM MeJA (JA+ACC) for 6 h. Actin8 was used as the internal control. Data are means (±sd) of three biological replicates. Lowercase letters indicate significant differences by one-way ANOVA analysis with SAS software (P < 0.05).