Mutations in EIN3 and EIL1 Repress the Enhanced Resistance against Necrotrophic Pathogen Botrytis cinerea in myc2.
(A) Symptoms on detached rosette leaves from 3-week-old plants of Col-0 (WT), myc2-2, ein3 eil1, and myc2-2 ein3 eil1 at day 2 after inoculation with mock or B. cinerea (B.c) spores.
(B) The lesion sizes on rosette leaves at day 2 after inoculation with B. cinerea spores. Data are means (±sd) of three biological replicates. Asterisks represent Student’s t test significance compared with the wild type (**P < 0.01).
(C) Quantitative real-time PCR analysis of ERF1, PDF1.2, and ORA59 in 12-d-old wild type, myc2-2, ein3 eil1, and myc2-2 ein3 eil1 treated with mock or 100 μM MeJA (JA) for 6 h. Actin8 was used as the internal control. Data are means (±sd) of three biological replicates. Different letters indicate significant differences by one-way ANOVA analysis with SAS software (P < 0.05). Capital letters correspond with each other, and lowercase letters correspond with each other.