Figure 6.
The Loss of Cytochrome b6f Complex Subunits and Biogenesis Factors Results from Their Increased Proteolytic Degradation.
The fate of cytochrome b6f subunits and biogenesis factors, LAO1, Rubisco, and PTOX2, was assessed as described in Figure 1 by immunoblots using the antibodies against those proteins listed between the two panels in two strains both expressing the tagged version of MCA1 and either defective for FtsH activity (mH ftsh1-1; right panel) or showing a 4-fold reduced accumulation of the Clp protease (mH {ClpP-AUU}; left panel). The β-subunit of the chloroplast ATP synthase is shown as a loading control.