Figure 7.

Cytochrome b₆f Subunits and Biogenesis Factors Are Preserved When Mitochondrial Respiration Is Impaired.

The accumulation of cytochrome b₆f subunits and biogenesis factors, LAO1, Rubisco, and PTOX2, was studied as in Figure 1A in WT-S24 and tmFH8 strains deprived of nitrogen sources under low light, either in the absence of reduced carbon in the medium (-Ac; left panel) or in anaerobic conditions (-O₂; cultured with gentle shaking, 50 rpm, in sealed Erlenmeyer flasks; middle panel), or in the respiratory mutant dum22 grown under 80 µE⋅m⁻²⋅s⁻¹ (right panel). The antibodies used are directed against those proteins indicated on the left of the figure, and the accumulation of PsaA provides a loading control.