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. 2014 Jan 21;26(1):373–390. doi: 10.1105/tpc.113.120113

Figure 1.

Figure 1.

Revertants Recovered the Wild-Type Level of b6f Complexes.

(A) Phototrophic growth on minimal medium and mixotrophic growth control on acetate medium at 10 µE m−2 s−1.

(B) Total cell proteins of the wild type, ccb2 and ccb4 mutants, and Rccb2-303, Rccb2-306, and Rccb4-301 revertants grown in acetate medium at 6 µE m−2 s−1 were separated by SDS/urea-PAGE and analyzed by immunodetection with antibodies against cytochrome b6f subunits (cytochrome f and cytochrome b6) and CCB factors involved in the covalent binding of ci heme to cytochrome b6 (CCB2 and CCB4) and by heme peroxidase activity detection using tetramethylbenzamidine.