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. 2014 Jan 28;26(1):454–464. doi: 10.1105/tpc.113.120311

Figure 5.

Figure 5.

Pi Destabilizes PT2 Protein in Vivo.

(A) Wild-type transgenic plants expressing 35S:PT2 in 1 mM CHX were treated with (+) or without (−) 3.5 mM Pi.

(B) pho2 mutant plants expressing 35S:PT2 in 3.5 mM Pi and 1 mM CHX were treated with (+) or without (−) MG132.

(C) Wild-type transgenic plants expressing 35S:PT2 in 3.5 mM Pi and 1 mM CHX were treated with (+) or without (−) 50 μM MG132 (proteasome inhibitor). Plants were treated with 3.5 mM Pi and 1 mM CHX.

(D) Wild-type transgenic plants expressing 35S:PT2 in 3.5 mM Pi and 1 mM CHX were treated with or without 50 μM E64d (Cys protease inhibitor).

For (A) to (D), samples were taken at various time points, and PT2-HA protein levels were analyzed by protein gel blots using anti-HA antibody. Stained gel bands of the large subunit of Rubisco, rbcL, were used as a loading control.