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. 2014 Jan 28;26(1):454–464. doi: 10.1105/tpc.113.120311

Figure 6.

Figure 6.

NLA Targets PT2 but Not PT1 for Degradation in Vivo.

(A) Protein levels of PT1 were determined in wild-type transgenic plants carrying two transgenes, XVE:MYC-NLA (inducible) and 35S:PT1-HA. Plants were either treated or not with β-estradiol to induce NLA expression. Samples were taken at 0, 1, 3, and 6 h after induction, and the expression levels of PT1-HA and MYC-NLA were analyzed by protein gel blots. Stained gel bands of rbcL were used as a loading control. Two independent transgenic lines (no. 1 and 2) were analyzed.

(B) Wild-type transgenic plants carrying two transgenes, XVE:MYC-NLA (inducible) and 35S:PT2-HA, were used. Plants were treated with or without β-estradiol to induce the NLA E3 ubiquitin ligase. Proteins levels were analyzed by protein gel blots using anti-HA antibody. Stained gel bands of rbcL were used as a loading control. Two independent transgenic lines (no. 1 and 2) were analyzed.