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NLA-Mediated Degradation of PT2 Is Dependent on PHO2 in Vivo.
pho2 mutant transgenic plants carrying two transgenes, XVE:MYC-NLA (inducible) and 35S:PT2-HA, were used. Plants were treated with or without β-estradiol to induce the NLA E3 ubiquitin ligase. Proteins levels were analyzed by protein gel blots using anti-HA antibody. Stained gel bands of rbcL were used as a loading control. Two independent transgenic lines (no. 1 and 2) were analyzed.
