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. Author manuscript; available in PMC: 2014 Mar 24.
Published in final edited form as: Sci Transl Med. 2013 Mar 13;5(176):176ra33. doi: 10.1126/scitranslmed.3005661

Fig. 2.

Fig. 2

Recognition of the naturally presented RMF/A2 complex on the cell surface by ESK1 in a HLA-A0201 and WT1 restricted manner. Binding of ESK1 to human mesothelioma cell lines JMN (a) and MSTO (b), to human leukemia cell lines BV173 (c). K562 (d), BA25 (e), and SET2 (f) with 10 ug/ml ESK1. Binding of ESK1 to AML blasts was measured on gated CD34+/CD33+ cells from HLA-A2 positive (g) or HLA-A2 negative (h) patients. Isotype human IgG1 was used as negative control. HLA and WT1 phenotype is shown for each cell type. Indirect flow cytometry was used in a–e and direct flow cytometry in f–h.