Figure 2. .

Transgenic labeling of tissues and cell types in the zebrafish heart using GFP reporter genes. (A) The different cell types of the zebrafish heart are GFP-labeled by transposon mediated insertional transgenesis. (A) Schematic depiction of a zebrafish heart indicating the route of blood flow from the sinus venous, through the atrium, ventricle and exiting from the bulbus arteriosus. GFP expression present in the three cardiac layers - (B,E) endocardium, (C) myocardium and (D, F) epicardium as well as examples of transgenes with a region-specific expression pattern including the (G) ventricle, (H) atrium, (I) bulbus arteriosus and (J-L) valves. Expression patterns are shown in the (B-D, I) embryonic, or (E-F,J-L) adult heart. Abbreviations: A, atrium; AV, atrioventricular valve; BA, bulbus arteriosus; BV, bulboventricular valve; V, ventricle. From ¹¹ with permission.