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. Author manuscript; available in PMC: 2014 Mar 24.
Published in final edited form as: Stem Cells. 2012 Jul;30(7):1313–1326. doi: 10.1002/stem.1120

Figure 4. Numb isoforms differentially regulate Notch and AKT.

Figure 4

(A): Effect of Numb4 or Numb4d7 overexpression on cleaved Notch (NICD) expression in HEK293T cells and in human LN229 glioblastoma cells. (B): Hes1 reporter assay in HEK293T cells after overexpression of Numb4, Numb4d7, NICD, or a control vector. Data shown are mean ± SEM. *, p < .05, t test. (C): Effect of overexpression of Numb4, Numb4d7, or a control vector on AKT activation in primary human glioblastoma stem-like cells (GSCs). (D): Effect of Numb shRNA on AKT activation in primary human GSCs or in human U87 glioblastoma cells. (E): Effect of overexpression of Numb4, Numb4d7, or a control vector on cleaved Notch (NICD) and AKT activation in primary GSCs. EDTA was used to trigger Notch cleavage. Abbreviations: NICD, Notch intracellular domain; Numb4d7, Numb4 delta 7.