Skip to main content
. Author manuscript; available in PMC: 2014 Mar 24.
Published in final edited form as: Stem Cells. 2012 Jul;30(7):1313–1326. doi: 10.1002/stem.1120

Figure 6. Effect of Numb isoforms on EGFR expression.

Figure 6

(A): Effect of Numb4, Numb4d7, or a control vector on EGFR and PDGFRA expression in primary human glioblastoma stem-like cells (GSCs). (B): Effect of Numb4, Numb4d7, or a control vector on EGFR expression in human LN229 glioblastoma cells in the presence or absence of serum. (C): Effect of Numb knockdown on EGFR expression in human U87 glioblastoma cells in the presence or absence of serum. (D): Real-time PCR illustrating effect of Numb isoforms on EGFR mRNA expression in primary human GSCs. (E): Effect of Numb isoforms on EGFR expression in human GSCs in the presence or absence of the proteasome inhibitor, MG132. (F): Effect of Numb isoforms on EGFR expression in human GSCs in the presence or absence of the lysosome inhibitor, bafilomycin A1. (G): Effect of Numb isoforms on neural differentiation marker expression in GSCs in the presence or absence of the EGFR inhibitor, AG1517. Abbreviations: EGFR, epidermal growth factor receptor; Numb4d7, Numb4 delta 7; PCR, polymerase chain reaction.