Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 24;9(3):e85345. doi: 10.1371/journal.pone.0085345

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

(A) SiRNA claudin-2 transfection resulted in a near-complete depletion of claudin-2 expression as assessed by Western blot analysis (4 days post-transfection). (B) IL-6 caused an increase in claudin-2 protein expression. Knocking-down claudin-2 by siRNA transfection prevented the IL-6 induced increase in claudin-2 expression as assessed by Western Blot analysis. (C) siRNA induced knock down of claudin-2 prevented the IL-6 induced drop in Caco-2 TER. (n = 6). _*, p<0.001 vs. control; _**, p<001 vs. IL-6 treatment. (D) Claudin-2 depletion prevented the IL-6 induced increase in urea flux across Caco-2 monolayers. (n = 6). _*, p<0.001 vs. control; _**, p<001 vs. IL-6 treatment.