Skip to main content
. 2014 Mar 24;9(3):e85345. doi: 10.1371/journal.pone.0085345

Figure 7. Effect of mRNA transcription inhibitor on IL-6 induced increase in Caco-2 TJ permeability.

Figure 7

(A) IL-6 caused a time-dependent increase in claudin-2 mRNA expression as assessed by real-time PCR (n = 8). *, p<0.001 vs. control. (B) mRNA polymerase inhibitor actinomycin-D (100 µg/ml) prevented the IL-6 induced increase in claudin-2 protein expression as assessed by Western Blot analysis. (C) Pre-treatment with actinomycin-D prevented the IL-6 induced drop in Caco-2 TER (n = 4). *, p<0.001 vs. control; **, p<0.001 vs. IL-6 treatment. (D) Pre-treatment with actinomycin-D also prevented the IL-6 induced increase in urea flux in Caco-2 monolayers (n = 4). *, p<0.001 vs. control; **, p<0.001 vs. IL-6 treatment.