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. 2014 Mar 24;9(3):e90861. doi: 10.1371/journal.pone.0090861

Figure 1. PGF2α release by omental and subcutaneous fat cells.

Figure 1

(A) PGF2α release by subcutaneous and omental primary preadipocytes in response to TNF-α and/or IL-1β (preadipocytes stimulated for 24 h with 1 ng/ml TNF-α, 1 ng/ml IL-1β or both). Results are expressed as pg/ml*μg protein*24 h (n = 14), (B) PGF2α release by isolated subcutaneous and omental mature adipocytes in response to TNF-α and/or IL-1β (isolated mature adipocytes stimulated for 2 h with 1 ng/ml TNF-α, 1 ng/ml IL-1β or both). Results are expressed as pg/106cells*2 h (n = 12), (C) PGF2α release by subcutaneous and omental adipose tissue explants in response to TNF-α and/or IL-1β (explants stimulated for 24 h with 1 ng/ml TNF-α, 1 ng/ml IL-1β or both). Results are expressed as pg/ml*mg tissue*24 h. Data are presented as mean ± SEM. p≤0.05 for treatment-by-depot interaction in panel A and p≤0.05 for treatment effect in panels A and C. * p ≤ 0.05.