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. 2014 Mar 24;9(3):e91946. doi: 10.1371/journal.pone.0091946

Figure 3. Real Time PCR analysis for xenobiotics induced transcriptional changes in CYP genes in SH-SY5Y and U373-MG cells.

Figure 3

Fold changes in altered mRNA expression of CYP1A1 in SH-SY5Y cells (A) and U373-MG cells (B) following the exposure of 3-MC (1–12 h), MCP (1–12 h) and 3-MC (12 h) then MCP (1–12 h) groups. Fold changes in altered mRNA expression of CYP2B6 in SH-SY5Y cells (C) and U373-MG (D) cells following the exposure of CPA (1–12 h), MCP (1–12 h) and CPA (12 h) then MCP (1–12 h) groups. Fold changes in altered mRNA expression of CYP2E1 in SH-SY5Y cells (E) and U373-MG (F) cells following the exposure of ethanol (1–12 h), MCP (1–12 h) and ethanol (12 h) then MCP (1–12 h) groups. β-actin was used as endogenous control to normalize the data and xenobiotic exposure induced alterations in transcripts are expressed in fold changes (mean ± SE) compared with unexposed controls. * = p<0.05 and ** = p<0.01 in comparison to respective unexposed controls; while, # = p<0.05 and ## = p<0.01 in comparison to 3-MC, CPA and ethanol (12) exposure respectively.