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. 2014 Mar 24;9(3):e92244. doi: 10.1371/journal.pone.0092244

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© 2014 Chu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Human ovarian cancer cells OVCAR3 were induced to activate matriptase by pH 6.0 (or control) buffer. Cell lysates were immunodepleted with the mAb M69. Cell lysates of the non-activation control cells (N, lane 1), the pH 6 activated cells (A, lane 2), and the immunodepleted fraction (D, lane 3) were analyzed for matriptase species by Western blot using the M24 mAb. B. and C. The cells fractions (cell), the shed fractions (shed), and the activated matriptase-depleted shed fractions (deplete) of OVCAR3 (B.) cells and OV2008 cells (C.) were analyzed for tryptic activity. RFU stands for relative fluorescent units. D. OV2008 human ovarian cancer cells were induced to activate matriptase by pH 6.0 buffer. The shed fraction (lane 1) and the activated matriptase-depleted shed fraction (lane 2) were analyzed for matriptase gelatinolytic activity by gelatin zymography.