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. 2014 Mar 24;9(3):e92244. doi: 10.1371/journal.pone.0092244

Figure 7. Matriptase shedding is tightly coupled with zymogen activation.

Figure 7

A. HaCaT human keratinocytes were treated with pH 6.0 buffer (A) or PBS as a non-activation control (N) for 20 min. The cell lysates (Cell) and the shed fractions (Shed) were analyzed for total matriptase (Total MTP) using the mAb M24 and activated matriptase (Activated MTP) using mAb M69. B. HaCaT human keratinocytes were treated with pH 6.0 buffer to induce matriptase activation and shed fractions were collected at the indicated times thereafter and analyzed for matriptase using the mAb M24.