Figure 1. Non-random acrocentric fusion when telomeres are disrupted by various approaches.

(A) Immunoblot of HT1080 whole cell lysates selected for empty vector or shTRF2 retroviral vector for 11 or 15 days with puromycin. Blot shows TRF2 protein as a doublet and β-actin as a loading control. (B) Acrocentric fusions are non-randomly induced in HT1080 cells expressing a retroviral shTRF2 construct for 10 days and 3 weeks. In addition, treatment of cells with the double-strand break inducer zeocin also results in high numbers of acrocentric fusions. asterisk (*) in graph legend denotes observations from dnTRF2 expression previously reported in [9].