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| Strain | Description | Capsule | Pneumolysin |
| D39 | Wild type serotype 2 (NCTC 7466) | + | + |
| D39cps− | Mutant lacking capsule [33] | − | + |
| D39ply− | Mutant lacking pneumolysin [20] | + | − |
| D39cps−ply− | Mutant lacking both capsule and pneumolysin (current study) | − | − |
| 110.58 | Wild type nonencapsulated [34] | − | + |
| 110.58::D39cps | Mutant with serotype 2 capsule [19] | + | + |
