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. 2014 Mar 24;9(3):e92511. doi: 10.1371/journal.pone.0092511

Figure 1. Tumour microtissue formation.

Figure 1

SEM pictures were taken after ten days: Monocultures of A549 and SV80 were seeded in a ratio of 2500 cells/40 μl, whereas Colo699 monocultures were cultured in form of 1250 cells/40 μl. All co-cultures were seeded in a carcinoma cell: fibroblast ratio of 1∶2/40 μl (A549 co-cultures: 2500 cancer cells +5000 fibroblasts / Colo699 co-cultures: 1250 cancer cells +2500 fibroblasts). (A/B) A549 monocultures, (C/D) A549 co-cultures; (E/F) Colo699 monocultures, (G/H) Colo699 co-cultures; (I/J) SV80 monocultures. All co-cultures displayed a more homogenous and rounder microtissue surface compared to monocultures.