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. 2014 Mar 24;9(3):e92328. doi: 10.1371/journal.pone.0092328

Figure 1. HHV-6A and HHV-6B duplex ddPCR assay design and specificity validation.

Figure 1

(A) Primers were designed to amplify an 89 base pair region of U57, encoding the major capsid protein of HHV-6. The shared forward and reverse primers (in bold) amplify both HHV-6A and HHV-6B, while the probes are specific for each virus with a three base pair mismatch. The HHV-6A probe sequence is in blue, while the HHV-6B probe sequence is in green. (B) The probes distinguish HHV-6A and HHV-6B viral DNA with high specificity. The HHV-6A FAM-labeled probe binds HHV-6A DNA (blue droplets in left plot) but not HHV-6B DNA. Likewise, the HHV-6B VIC-labeled probe binds HHV-6B DNA (green droplets in right plot), but not HHV-6A DNA.