Figure 3. Collagen content in wildtype and uPARAP^-/- after wound injury.

(A) Hydroxyproline content was measured in wounds on day 8 and 12 post-injury. (B) Trichrome staining was done on fixed wound samples on day 8 and 12 after wounding. Representative images are shown from at least 4 mice/genotype (C) Ratio of collagen staining area versus total tissue in wound area was quantified in slides stained with trichrome. Data are presented as mean± SEM; n of mice in each group is indicated inside the bars. (D) α-SMA expression in wound tissue homogenates from day 8 post-injury was evaluated by Western blot analysis (top). GAPDH was used as a loading control (bottom), n = 4/genotype. (E) Intensity of bands on immunoblots were quantified and normalized to GAPDH. (F) Representative images of α-SMA staining on fixed wound samples on day 8 post-injury. (G) The area positive for α-SMA was quantified. Each point represents an individual mouse. (H) Area of granulation tissue was traced and quantified in trichrome stained wound samples from day 8. Data are presented as mean± SEM, * P<0.05 compared to wildtype.