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. 2014 Mar 24;9(3):e92664. doi: 10.1371/journal.pone.0092664

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© 2014 Gaignier et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Splenocytes were isolated from mice and labeled as described in the Methods section. For each mouse, the lymphocyte populations were determined as in the two examples presented here. (A) Splenocyte analysis by flow cytometry using FlowJo software and sorted with FSC/SSC profiles, separating viable lymphocytes (black arrow) from the other cells. (B) Among the lymphocyte population, CD19⁺ B cells (black arrow) were selected in the FSC/CD19 window and CD3⁺ T cells (white arrow) were selected in the FSC/CD3 window. (C) Among the lymphocyte population, CD3⁺CD8⁺ T cells (black arrow) were selected in CD3/CD8 profiles and CD3⁺CD4⁺ T cells (white arrow) were selected in CD3/CD4 profiles.