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. 2014 Mar 24;9(3):e92664. doi: 10.1371/journal.pone.0092664

Figure 7. Percentages of dividing lymphocytes 72 hours after mitogenic stimulation.

Figure 7

In vitro culture of splenocytes stained with CFSE allowed the analysis of B, Tc and Th divisions in response to a mitogenic stimulation. Subpopulations of lymphocytes were determined within the lymphocyte gate. Within each subpopulation, CFSE fluorescence was analyzed by flow cytometry. Then, the FlowJo software automatically determined generation 0 and calculated the percentage of dividing cells from the intensity of residual CFSE in cells. Each group was compared to the others. n = 5 mice per group. Differences were found to be statistically significant using an ANOVA and Tukey post-hoc test. ** p<0.005; *** p<0.001. (A) Percentages of dividing CD19+ B cells in response to LPS. Dividing B cells were significantly decreased in the hindlimb unloaded (HU) group compared to the restrained (R) and control (C) groups (by 33% and 40%, respectively). (B) Percentages of dividing CD3+ T cells, CD3+CD8+ Tc cells and CD3+CD4+ Th cells in response to ConA. No significant difference was obtained for dividing T cells despite the decreases observed for the HU group compared to C mice.