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. 2014 Mar 24;9(3):e92678. doi: 10.1371/journal.pone.0092678

Figure 3. Ectopically expressed SARS-MRV μ1 and σ1 induced typical apoptosis in 293 T cells.

Figure 3

(A) Cells transfected with pEGFP-C3–σ1 or pEGFP-C3–μ1 had a significantly higher rate of apoptosis than cells transfected with the pEGFP-C3 control vector (p<0.01), detected with flow cytometry at 18 h posttransfection. (B–F) Electron microscopic images of 293 T cells 48 h after transfection with pEGFP-C3 (B), pEGFP-C3–σ1 (C–D), or pEGFP-C3–μ1 (E–F) identified apoptotic cells with early chromatin condensation (C, E) and late karyorrhexis (D, F). Bar  = 2 μm (E) or 5 μm (B, C, D, F).