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. 2014 Mar 24;9(3):e92783. doi: 10.1371/journal.pone.0092783

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© 2014 Skočaj et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Domain structures and tags of the fluorescently fused OlyA and PFO. (B) Western blotting of recombinant OlyA variants expressed in bacteria. H₆, hexa-histidine; EGFP, enhanced green fluorescent protein; other abbreviations as used in the main text. Calculated relative molecular masses: Mr: OlyA-mCherry-H₆, 44,318.58 Da; H₆-mCherry-OlyA, 43,409.66 Da; OlyA-mCherry, 42,489.69 Da; mCherry-OlyA, 42,035.15 Da. Detection of the proteins in Western blotting was performed using polyclonal anti-OlyA antibodies. Arrows denote spontaneously cleaved mCherry-tagged OlyA products [46].