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. 2014 Mar 24;9(3):e92783. doi: 10.1371/journal.pone.0092783

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© 2014 Skočaj et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–F) Representative images of co-localisation between OlyA-mCherry (red) and other membrane markers (green), as indicated. Scale bar, 5 μm. (G) Quantitative analysis of the co-localisation between OlyA-mCherry and the other membrane markers. Pearson's correlation coefficients were calculated using the JACoP plugin (Image J programme) from optical sections. The degree of co-localisation from the Pearson's value correlation coefficients s were categorised as very strong (0.85 to 1.0), strong (0.49 to 0.84), moderate (0.2 to 0.48), weak/moderate (0.1 to 0.2), weak (−0.26 to 0.09), and very weak (−1 to −0.27). Error bars are the standard errors of the means (n = 5).