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. 2014 Mar 24;9(3):e92783. doi: 10.1371/journal.pone.0092783

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© 2014 Skočaj et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–H) Representative fluorescent images of MDCK cells (blue, DAPI; red, OlyA-mCherry) showing immunolabelling of respective membrane domains (green, as indicated) and labelling of OlyA-mCherry (1 μM). After 5 min, OlyA-mCherry shows “weak/moderate” co-localisation with clathrin on the plasma membrane (A), and “moderate” with caveolin-1 (B) and EEA-1 (C). After 30 min, OlyA-mCherry shows “strong” co-localisation with caveolin-1 (D) and “weak/moderate” with cathepsin L (E). After 90 min, OlyA-mCherry shows “moderate” co-localisation with the GM130 (F) and P230 (H) markers of the Golgi apparatus, and “weak/moderate” for giantin (G). (I) Quantitative analysis of the co-localisation between OlyA-mCherry and the other membrane markers. Co-localisation in (F–H) are restricted to individual spots. Scale bars: 20 μm.