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. 2014 Mar 24;9(3):e93119. doi: 10.1371/journal.pone.0093119

Figure 1. Fn chitinase affects the biophysical properties of the biofilm.

Figure 1

Fn was grown to mid-log phase prior to the analyses. (A) The relative hydrophobicity of WT and chi mutants assayed by phenyl-sepharose column chromatography (HIC) and microbial adhesion to the nonpolar solvent hexadecane. *P<0.05, **P<0.01, and ***P<0.001 compared to WT (n = 6). (B) Autoaggregation of WT and chi mutants in PBS assayed at 24 and 48 h. *P<0.05, and **P<0.01 compared to WT (n = 6). (C) Size distribution for planktonic cultures of the strains in PBS measured by qNano analysis. (D) Particle translocation time (fwhm). The chi mutants had a larger fwhm duration than that observed for WT, indicating that the lower charge chi mutants took longer to traverse the pore. Mean presented in dots was calculated from every 100 data points.