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. 2014 Feb 28;111(11):4179–4184. doi: 10.1073/pnas.1401727111

Fig. 5.

Fig. 5.

(A) Cellular localization of the NRAS isoforms. Displayed are single-channel and merged confocal micrographs of COS-7 cells ectopically expressing the NRAS isoforms and NRAS G12D mutant. Whereas isoforms 1, 2, and 4, and the NRAS G12D mutant were located only in the cytoplasm, isoforms 3 and 5 displayed nuclear localization as well. Pink nuclei show nuclear isoform localization (green arrows). Blue nuclei show no nuclear isoform localization (white arrows). The white dashed boxes in the merged pictures indicate the magnified areas. (B) Nuclear localization of NRAS isoforms 3 and 5. Three-dimensional images of cells transfected with NRAS isoforms 3 and 5 (Z-stacks). Z-stacks were collected (0.2 μm per slice) and images were chosen from the middle of the nuclei. Side views of the cells (across bottom and side of figures) are also shown. Both isoforms show nuclear localization (pink).