Fig. 5.

Autophagy and apoptosis are induced after Sudestada1 knockdown. Expression of a sud1 (B) but not of a white (control) (A) RNAi at the wing disc posterior compartment provokes nucleation of ATG8-GFP expressed at the same compartment, revealing that autophagy was induced. Autophagy induction was confirmed by Lysotracker-positive staining (C and D), as well as by nucleation of Lamp1-GFP (E and F) in cells of the same disc territory. Note in E and F that small Lamp1-GFP foci become bigger after sud1 RNAi treatment. (G–J) Apoptosis is triggered after sud1 silencing: Cells of the posterior wing disc compartment expressing sud1 (H and J) but not those expressing a white (control) RNAi (G and I) become TUNEL positive (the arrows in H show two examples of TUNEL-positive cells). The posterior disc compartment that expresses Engrailed (green staining in I and J) is marked with a dotted line.