Fig. 2.
Effect of stalling an RNAP upstream of a CPD lesion. (A) DNA substrate. A single CPD was present in the template strand. (B) Patch synthesis assay monitoring repair of a single CPD in the template strand. Additions are as described for Fig. 1. (C) Quantification of the [32P]-dATP incorporated into the template strand of B3-CPD-TS during patch synthesis assays, normalized to the amount incorporated into the UV-irradiated pHWL1-2 control in each lane. Values are the average of at least three repeats and are shown with SD.