Fig. 3.

Effect of a protein roadblock on TCR at a distance. (A) DNA substrate. Plasmid pHWL1-T7A1-2lacO contains a lac operator between the T7A1 promoter and a repair-reporter cassette. DNA lesions were introduced randomly into both strands of the plasmid by exposure to 30 J/m² of UV light. (B) Quantification of the [³²P]-dATP incorporated into the template and nontemplate strands of the pHWL1-T7A1-2lacO reporter cassette during patch synthesis assays normalized to the amount incorporated into the UV-irradiated pHWL1-2 control in each lane. Additions are as described for Fig. 1. Values are the average of at least three repeats and are shown with SD. A sample dataset is shown in Fig. S3.