Fig. 5.
Effect of pausing RNAP upstream of a nonstalling lesion. (A) DNA substrates. (B) Patch synthesis assay monitoring repair of a bio-dT in the template strand in a substrate containing an ops pause site (B3ops-bio-TS) or a control lacking the ops pause site (B5-bio-TS). Additions are as described for Fig. 1. (C) Quantification of the [32P]-dATP incorporated into the template strand of each substrate during patch synthesis assays normalized to the amount incorporated into the UV-irradiated pHWL1-2 control in each lane. Values are the average of at least three repeats and are shown with SD.