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. 2014 Feb 10;111(11):3955–3960. doi: 10.1073/pnas.1322937111

Fig. 4.

Fig. 4.

Effects of apoE on gene silencing and cell uptake. (A) Silencing effects of apoE on cKK-E12, cKK-A12, and cKK-O12 LPNs in HeLa cells (siRNA: 50 ng per well). In the presense of apoE, the order of silencing effects was cKK-E12 > cKK-A12 > cKK-O12, correlating well with in vivo activity. (B) Cellular internalization of cKK-E12 with Alex-647–labeled siRNA after 3 h of incubation is demonstrated by HT automated confocal microscopy (Alex-647 is pseudocolored green). ApoE enhanced cell uptake and endosomal escape of cKK-E12. (Scale bar: 20 μm.) (C) In vivo factor VII gene-silencing effects of cKK-E12 in wild-type apoE−/− and LDLR−/− mice. Data points are expressed as a percentage of PBS control animals and represent group mean ± SD (n = 3; ***P < 0.005; NS, not significant; t test, double-tailed). Control, PBS; WT, wild type.