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. 2014 Mar 3;111(11):4055–4060. doi: 10.1073/pnas.1323285111

Fig. 2.

Fig. 2.

Oligomeric states of Rocext in solution. (A) Size-exclusion chromatography elution profile of purified Rocext showing two peaks (48 and 24) that correspond to molecular masses of ∼48 and ∼24 kDa, respectively, based on standard calibration curve. (B) SDS/PAGE of peaks from A, 48 and 24. Molecular mass standards are labeled in kilodaltons to the left of the marker lane (MW). (C) Western blot of peaks from A, 48 and 24. Molecular mass standards are labeled in kilodaltons to the left of the marker lane (MW). (D) SEC-MALS profile of purified Rocext showing two peaks [48 (black solid line) and 24 (black dashed line)], along with their average molecular mass calculated from light scattering (blue and red lines, respectively). (E and F) MALDI-TOF MS spectra of 48 and 24, respectively.