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Conversion of Roc48 to monomers upon guanine nucleotide binding. (A) Size-exclusion chromatography profile of Roc48 apo state (green, ∼48 kDa), GDP-bound (blue, ∼24 kDa), and GppNHp-bound (red, ∼24 kDa) shows conversion of the Roc48 peak into Roc24 peaks upon binding to either GDP or GTP. (B) SEC-MALS profile of GDP- and GppNHp-bound Rocext with calculated molecular masses of 25 kDa for both (black line on the peak).
