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RocWT and RocR1441H thermal stability determined by ThermoFluor assay. (A) Melting curve showing that RocWT (opened circle) and RocR1441H (X) have similar Tm values in nucleotide-free forms. (B) Melting curve of GDP-bound RocWT and RocR1441H showing similar thermal stability. (C) Melting curve of GppNHp-bound Roc showing that RocR1441H is more thermally stable than RocWT, indicative of a higher affinity of RocR1441H for GTP.
