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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Methods. 2013 Jun 25;66(2):131–138. doi: 10.1016/j.ymeth.2013.06.021

Table 2.

Förster distances (R0) for common fluorescent protein FRET pairs.

FP FRET-Pair R0 Refs.
ECFP-EYFP 4.7 a
ECFP-Citrine 4.8 b
mCerulean–mCherry 4.8 a
ECFP-Venus 5.0 b
mCerulean-EYFP 5.0 a
Cerulean–Venus 5.2 b
mCerulean–mVenus 5.3 a
SECFP-SEYFP 5.4 b
EGFP-mCherry 5.4 b
TagGFP-TagRFP 5.7 b
mTFP1-Citrine 5.7 b
mTFP1-mOrange 5.7 b
Citrine-mKate2 5.8 b
Clover-mCherry 5.8 b
mVenus-mCherry 5.8 a
mVenus-mOrange 5.8 a
mTurquoise1-SEYFP 5.8 b
mTurquoise2-SEYFP 5.9 b
Clover-mRuby2 6.3 b
a

Kremers and Goedhart [33].

b

Lam et al. [34].