(A) Lcn2, S100a8 and S100a9 were detected by quantitative real time PCR in isolated colonic crypts of WT mice and Il22−/− mice 48h after infection with WT S. Typhimurium. Infected WT n=6, infected Il22−/− n=6, mock n=2. Data are expressed as fold increase over mock-infected WT mice. Data represent the geometric mean ± standard error (for some conditions error marks are not visible due to small error). A significant increase over mock control is indicated by ** (P value ≤ 0.01) and *** (P value ≤ 0.001). (B) Lcn-2, S100A8, S100A9, myeloperoxidase (MPO), and tubulin were detected 48h post infection by immunoblot in isolated crypts of WT and Il22−/− mice that were mock-infected or infected with S. Typhimurium.